Abstract
Introduction
Material & Methods
Results
Discussion

Acknowledgements

NSF Student Research

Figure 1- 4 I Figure 5- 7

Matt Harris: Preparation Of Apo-Metallothionein For Use in Metal Turnover Analysis

 


 

Figure 5.  ICP-MS chromatogram of 4.46 µg apo-metallothionein showing 63Cu, 64Zn, and 114Cd
 
 

Figure 6.  ICP-MS chromatogram of 4.46 µg apo-metallothionein showing 95Mo, 98Mo, and 200Hg
 
 

Metal ng metal/µg protein
Copper 3.00
Zinc 0.00
Molybdenum 0.00
Cadmium 8.33
Mercury 0.00
Metal ng metal/µg protein
Copper 92.23
Zinc 100
Molybdenum 100
Cadmium 97.01
Mercury 100
Table 2.  ng of metal/µg of apo-metallothionein Table 3.  Percent Reduction of Metal in apo-metallothionein corrected for concentration

 
 

Figure 7.  ICP-MS chromatogram of direct FIA injection of 4.46 µg of apo-mt showing all metals analyzed for

Figure 1- 4 Figure 5- 7