Fig 1.  Overlay of UV Size Exclusion Profile of MT at 254 and 280 nm
 
 
 

Figure 2.  ICP-MS chromatogram of 10 µg metallothionein standard showing 63Cu, 64Zn, and 114Cd.  The small peaks before 10 minutes and after 25 minutes represent known quantities of analyte injected into the eluent stream by FIA.  The integrated areas were used to quantify the metal content of the protein. 
 
 

Figure 3.  ICP-MS chromatogram of 10 µg metallothionein standard showing 95Mo, 98Mo, and 200Hg
 
 
 
 
 

Table 1.  ng of metal/0.5ug of metallothionein
 

Fig 4.  Overlay of UV Size Exclusion Profile of apo-MT at 254 and 280 nm.  There has been a significant decrease in absorption at 254 due to removal of the metal-mercaptide bonds (cf. figure 1)