Kelly Thrippleton:
Purification, Isolation and Molecular
Analysis of Apo-Hemocyanin from the Garden Snail, Helix
aspersa, Using Potassium Cyanide and Coupled HPLC/ICP-MS
Figure 2a.Tandemly coupled
HPLC/ICP-MS
chromatograms showing Cu associated with a
25 µl injection of Helix aspersa hemocyanin.
Flow
Injection Analysis (FIA) of 40ng of Cu was used to quantify the copper
content of
the protein.
Figure 2b. Tandemly coupled
HPLC/ICP-MS
chromatograms showing Cu associated with a 25 µl injection of
apo-hemocyanin.
Flow
Injection Analysis (FIA) of 40ng of Cu was used to quantify the copper
content of the protein.
Figure 2c Tandemly coupled
HPLC/ICP-MS
chromatograms showing Zn and Cd associated
with a 25 µl injection of Helix aspersa hemocyanin.
Flow Injection Analysis (FIA) of 40ng of both Zn and Cd were used to quantify
zinc and cadmium content of the protein.
Figure 2d Tandemly coupled
HPLC/ICP-MS
chromatograms showing Zn and Cd associated with a 25 µl injection of
apo-hemocyanin.Flow Injection Analysis (FIA) of 40ng of both Zn and Cd were used to quantify
zinc
and cadmium content of the protein.
