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A. COURSE OBJECTIVES
The aim of this course is to demonstrate the utility of electron microscopy
as a basic research technique for biological investigation and research.
To successfully complete the course and obtain a good grade, a student must
not only be adept at microscope operation and tissue preparation but also
micrograph interpretation and data presentation.
The initial part of the
laboratory component of this course is designed to introduce students to
the theory and practice of operation of the transmission electron microscope
(TEM) and supporting equipment (e.g. ultramicrotome and knife maker), the
scanning electron microscope (SEM) and supporting equipment (critical point
dryer and sputter coater) and darkroom equipment.
The theory of scanning
transmission electron microscopy and analytical electron microscopy including
energy and wavelength dispersive X-ray microanalysis and electron energy
loss spectroscopy will also be outlined in the lectures. Specimen preparation
and evaluation will also be discussed at great length.
By the latter part
of the course, each student is expected to be sufficiently familiar, facile
and proficient with the various techniques in electron microscopy to allow
him/her to individually conduct and complete a research project which utilizes
the virtues of the technique.
PUBLICATION ON THE WORLD-WIDE
WEB
Students enrolled in the class are also expected to provide a copy of their
projects as a World Wide Web document. As part of the class, students will
be taught the process of digitizing and manipulating images and incorporating
them into hyperlinked documents, with annotated text using HTML. Examples
of previous student projects can be obtained by clicking on the button bar.
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