Course Objective
Class Syllabus
Requirement
Exams
Lab Information
Lab Syllabus
Class Policy
Bio 211A I Bio 340 I Bio 464I Bio 468/568
Bio 490 I

B2. LABORATORY SYLLABUS:  BIOL 211A, BIOLOGICAL SCIENCE I, Fall 2007

REQUIRED TEXT: Laboratory Manual for Biological sciences I.  Mason, A.Z., Carlberg, D., Gharakhanian, E.,  Brusslan, J. and Palmier, C. 2004. 7th Edition.  Pearson Custom Publishing.  ISBN# 0-536-80364-1.  

WEEK  DATE * LABORATORY EXERCISE
Sept 3/4  No Laboratory (Labor Day).
  Sept 5/6   Introduction, Safety, Orientation, Units of Measurement
Sept 10/11  Instrumentation:  Light Microscope, pH meter, Balance, Pipettor
  Sept 12/13  Spectroscopy: Absorption Spectra Molar Extinction Coefficient
Sept. 17/18  Light Microscopy & Electron Microscopy
  Sept. 19/20 Cellular Fractionation
Sept. 24/25 Protein Quantification of Cellular Fractions
  Sept. 26/27   Enzyme Markers of Fractional Purity
Oct. 1/2 Enzyme Kinetics: Determining Km and Vmax
  Oct. 3/4 LABORATORY PRACTICAL EXAM I: Manual check
6 Oct. 8/9  Anaerobic Respiration: Fermentation (Report) Fire Drill 1.30 p.m.
  Oct. 10/11 Aerobic Respiration (Fire Drill 1:15 p.m.)
Oct. 15/16 

Library workshop & F1 cross for Mend. Genetics

  Oct. 17/18 Photosynthesis: Plant Pigments
Oct. 22/23  Photosynthesis: Hill Reaction (remove adult flies)
  Oct. 24/25 Mitosis & Meiosis
Oct. 29/30  Mendelian Genetics: Drosophila Genetics. 1st Draft report due
  Oct. 31,Nov.1 SDS PAGE
10  Nov. 5/6 Differential Gene Expression
  Nov. 7/8  LABORATORY EXAM II 
11 Nov. 12/13 Veteran's Day- Campus Closed
  Nov. 14/15 Bacterial Culture Techniques/ Lac Operon
12 Nov. 19/20 Examine Cultures, Isolation of Plasmid DNA
  Nov. 21-23 NO LABORATORY:  THANKSGIVING HOLIDAY
13 Nov. 26/27  DNA Restriction Analysis by Gel Electrophoresis
  Nov. 28/29 Restriction Analysis data analysis and PCR Preparation
14 Dec. 3/4 PCR Gel, start PCR applications.
  Dec. 5/6 PCR gel results; run gel on PCR applications; Transformation
15 Dec. 10/11 Transformation results & Natural Selection
  Dec. 12/13  EXAM III. CHECK OUT OF LABORATORY

* Listed dates represent laboratories undertaking same exercise.

Various additional exercises, handouts and supplementary material to compliment these laboratory exercises can be downloaded directly from the links in the syllabus website.