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Misty Borja: Estimation of Cu
Turnover in The Visceral Complex of Littorina littorea
By Isotopic Ratio Analysis Using ICP-MS. |
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ESTIMATIONS OF Cu TURNOVER IN THE VISCERAL COMPLEX OF Littorina littorea BY
ISOTOPIC RATIO ANALYSIS USING
ICP-MS.
M. Borja and A. Z. Mason, Ph.D.
Department
of Biological Sciences, California
State University, Long Beach, CA.
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The purpose of the present study was to assess the potential of a new technique involving the sequential coupling of size exclusion (SE)
and an ion exchange (IE) HPLC systems to an Inductively Coupled Plasma Mass Spectrometer
(ICP-MS) for studying metalloprotein speciation and identifying imbalances in metal homeostasis.
This procedure chromatographically fractionates samples based upon size
and charge while simultaneously identifying and quantifying the isotopic abundance of associated metals. In addition to being highly
selective and sensitive, this technique has the added virtue that, because elemental identification is based upon mass, it can monitor the
long-term movements of certain metabolically important metals, such as Cu, which have multiple stable isotopic forms and can not be
studied conventionally because of a lack of convenient radio tracer. The capabilities of this new technique are illustrated by examining
the cytosolic distribution of Cu and other metals within the visceral complex of the marine mollusc
Littorina littorea.
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