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H. PROPOSED LABORATORY COURSE OUTLINE BY CLASS:
1) Introduction, Laboratory Tour, Safety, Prep. solutions for TEM.
2) Specimen preparation for TEM. Video Presentation.
3) Trimming, Glass Knife Preparation, Microtomy I
4) Microtomy II: Semi-thins. Light Microscopy.
5) Microtomy III: Semi/Ultra-thins.
6) Microtomy IV: Staining for TEM.
7) PRACTICUM I
8) TEM Operation I. Basic Principles; HT, Filament, Gun Alignment.
9) TEM Operation II. Condenser/ Objective astigmatism Correction, OUF.
10) Trip to USC EM center. * EDX, WDS, EELS AUGER. STEM, Stereo SEM.
11) TEM Operation III. Use Of kV, Objective/condenser/intermediate apertures.
12) Photography, Developing/Loading Film.
13) Printing and Enlarging.
14) PRACTICUM II.
15) ABSTRACTS DUE. Introduction to the SEM.*
16) Specimen Prep for SEM; Conventional; Cryofracture.
17) Critical Point Drying, Sputter Coating.
18) SEM Operation I.*
19) SEM Operation II.*
20) EDX Video Presentation
21) Specialized TEM Techniques I; Celloidin/Formvar Films, Negative Staining.
22) Specialized TEM Techniques II; Ultracryomicrotomy/Ultracytochemistry Ultra-immunocytochemistry.
23) Specialized TEM Techniques III; Selected Area Diffraction.
24) PRACTICUM III.
25) Student Discussions of Research Project.
26) EM Project.
27) EM Project.
28) EM Project.
29) EM Project.
30) EM Project.
31) FINAL EXAM (Theoretical aspects of electron microscopy).
*Demonstration of equipment at USC may be outside normal class hours. Demonstration
of equipment subject to availability.
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