The Guide for the Care and Use of Laboratory Animals
Issues 1 - 6
The Guide sets forth the following matters for institutions to consider in the development of research protocols.
- Rationale and purpose
of the proposed use of animals.
- Justification of
the species and number of animals requested. Whenever possible, the number of animals requested should be justified statistically.
- Availability or appropriateness of the use of less-invasive procedures, other species, isolated organ preparation, cell or tissue
culture, or computer simulation (see Appendix A, "Alternatives").
- Adequacy of
training and experience of personnel in the procedures used.
- Unusual housing and husbandry requirements.
- Appropriate sedation,
analgesia, and anesthesia. (Scales of pain or invasiveness might aid in the preparation and review of
protocols; see Appendix A, "Anesthesia, Pain and Surgery.")